THE 4 CORNERSTONES OF ADC DESIGN.

Targeting Molecule

  • Antibodies (IgG1,2,4)
    • Human(ized)
    • Chimeric
    • Murine
  • Antibody Fragments
    • Minibodies
    • FAB / FAB2
    • scFV
  • Peptides
    • Linear
    • Cyclic
  • Proteins
  • Small Molecules

Toxins

  • Auristatins
  • Maytansines
  • Duocarmycins
  • PBD Dimers
  • Doxorubcin
  • Nemorubicin
  • Paclitaxel
  • Amanitins
  • Camptothecin

Conjugation Chemistry

  • Cysteine
    • Native hinge reduction
    • Engineered cysteine – addition/deletion
    • Disulphide bridging
  • Lysine
    • SMCC, SPDP, SPDB, 2-IT
    • Toxin-SH, Toxin-NHS
    • Two-step, one-step, one-pot
  • Enzymatic
    • Sortase
    • FXIII and BTG
    • Glycan remodelling
  • Protein Ligation
    • Inteins

Linker Customisation

  • Tether
    • Maleimide – SH
    • NHS – Lysine reactive
    • Click – DBCO, BCN, Azide and Alkyne
  • Polarity
    • PEG
    • Peptide – GLY3
    • Phosphates
    • Pyrophosphates
  • Trigger
    • Protease
    • Reduction
    • Glucuronide
    • Phosphodiesterase
  • Immolation
    • PABC
    • DMAE
    • MAC
    • Phosphate